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Ulated based on normalized, log2-transformed gene expression values from the

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The current study focused on de novo RNA sequencing of the salivary glands of both Ddle line). In the presence in the nocodazole, the distribution of EBN-producing swiftlets and non-EBN-producing swiftlets. BMC Genomics (2017) 18:Page 9 ofTable 4 Expression profiles of genes of potential importance in salivary gland development and EBN compositionsGene function Salivary development Gene CREB3L2 SEC63 SEC24A SAR1 AA versus Aerodramus species Gene function EGFR signalling pathway Gene ARF4 BCAR1 CAV1 CEBPA ELF3 EPS15 EPS8 JUN PLEC RAB5A REPS1 ZPR1 VAV2 STAT3 N-glycan biosynthesis ALG2 MGAT1 RPN1 FUT8 RPN2 AMC versus AFC Gene function EGFR signalling pathway Gene JUND HDAC BCAR N-glycan biosynthesis Alanine and Aspartate metabolism MGAT GOT1 PDHB AFM versus AFC Gene function MAPK signalling pathway BMP signalling pathway Fatty acid synthesis Gender comparison of AFC Gene function N-glycan trimming Gene EDEM2 Male 3.951 Female 0.359 log2 3.459 q-value 0.008 Gene HMGN1 TOB2 ACADVL AFM 23,486.800 20.463 10.909 AFC 2685.98 0.959 50.803 log2 -3.128 -4.414 2.219 q-value 0.001 0.001 0.001 AMC 4.911 1.357 7.776 20.278 226.741 894.023 AFC 39.541 580.771 200.736 256.555 7.0263 135.641 log2 3.009 8.740 4.690 3.661 -5.012 -2.721 q-value 0.001 0.001 0.001 0.046 0.000 0.000 AA 0.683 6.407 0.695 8.626 6.816 0.198 0.754 0.411 6.047 17.934 6.795 1.079 49.553 2.453 6.546 4.617 66.407 13.173 1.069 Aerodramus spp 48.954 51.836 50.207 49.820 603.607 10.035 13.668 11.504 31.197 47.435 21.529 13.242 239.912 53.308 90.860 91.067 552.366 84.076 71.026 log2 6.162 3.016 6.174 2.529 6.468 5.657 4.178 4.803 2.367 1.403 1.663 3.617 2.275 4.441 3.794 4.301 3.056 2.674 6.053 q-value 0.026 0.003 0.026 0.018 0.001 0.026 0.026 0.021 0.005 0.046 0.040 0.001 0.011 0.001 0.001 0.004 0.003 0.003 0.001 AFM 65.608 36.723 15.763 38.916 AFC 76.666 34.793 15.414 23.855 AMC 51.669 50.081 19.240 16.996 AA 0.000 5.926 19.20 6.AFC Aerodramu.Ulated based on normalized, log2-transformed gene expression values from the nCounter data and the RNA-Seq FPKM data of the 36 genes. The mean correlation was 0.54 with a 95 bootstrap confidence interval.DiscussionCharacterization of the swiftlet transcriptomeWith the advancements in sequencing technologies, the genomes of several avian species, including Peking ducks [30], passenger pigeons [15], and vultures [31], have been sequenced and characterized, whereby this inclusion has accelerated the research PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28724915 on functional genomics and improved our understanding of the genetic regulation of important traits. However, for some non-model organisms and minor species, it is not feasible to perform whole genome sequencing because of the expensive cost. Sequencing based on NGS technology has provided an opportunity to use a transcriptomic approach to study mechanisms that control certain traits in non-model organisms [32]. It is generally recognized that EBN is one of the most expensive animal-based products; it is often known as the "caviar of the East" [4]. Nevertheless, the transcriptomic profiles of swiftlets have not been established. The current study focused on de novo RNA sequencing of the salivary glands of both EBN-producing swiftlets and non-EBN-producing swiftlets. The transcriptomic sequencing was completed using the Illumina Genome PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28388412 Analyzer system platform, HiSeq2000, with a 100 bp paired-end read. A paired-end sequencing protocol was selected for this study because it is critical for the assembly of short (