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Sin) in P. fluorescens SBW25 (De Bruijn Raaijmakers, 2009a), MassAandMassBC(massetolide

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In numerous CLP- roducing Pseudomonas strains, a GacA box is presp entupstreamtheLuxRregulatorsflankingtheCLPbiosynthesisgene clustersuggestingthatotherCLP- roducingPseudomonas Levels are elevated in brain synaptosomes of heterozygous strains could p show a comparable regulation of lipopeptide biosynthesis (Song, Voort, etal.,2015).Besides the GacA/GacS regulatory system, N- cylhomoserine a , enhanced by editing and by alternative splicing lactone (N- HL)- ediated quorum sensing was shown to be reA m quiredforviscosinandputisolvin biosynthesis(Cui, Harling,Mutch, Darling,2005;Dubern,Lugtenberg, Bloemberg,2006)inP. putidastrainPCL1445,twoheatshockproteinsDnaKand DnaJlocateddownstreamoftheGacsystemwereshowntoregulate putisolvinbiosynthesis(Dubern,Lagendijk,Lugtenberg, Bloemberg, 2005).RecentstudiesonthegeneticregulationofmassetolideAbiosynthesis in P. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24876777 fluorescens SS101 revealed that the serine protease ClpP collectively together with the chaperone ClpA regulates the biosynthesis of massetolidesvia a particular pathway involving the LuxR regulator (MassABC),theheatshockproteinsDnaKandDnaJ,andproteinsof the tricarboxylic acid (TCA) cycle (De Bruijn Raaijmakers, 2009b; Song,Aundy,vandeMortel, Raaijmakers,2014;Song,Sundqvist, etal.,2015). Pseudomonas sp. CMR12a is actually a biocontrol strain isolated from the cocoyam rhizosphere in Cameroon (Perneel etal., 2007).This strain producestwoclassesofCLPsnamelyorfamidesandsessilinstogether with two kinds of phenazines, phenazine- - arboxylate (PCA) and 1c phenazine- - arboxamide (PCN) (D'aes etal., 2014; Perneel etal., 1c 2007). Orfamides are also made by biocontrol agents belonging to the P. protegens group (Gross etal., 2007; Jang etal., 2013; Ma, Geudens,etal.,2016;Takeuchi,Noda, Someya,2014),whilesessilins are structurally connected towards the tolaasins produced by the mushroom pathogen, P. tolaasii. Sessilins are essential for biofilm formation, when orfamides are PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23387799 essential for the swarming motility of CMR12a (D'aesetal.,2014)andbothCLPsareimportantforbiocontrol(D'aes etal., 2011; Hua H te, 2015; Ma, Hua, Ongena, H te, 2016; Olorunleke,Hua,Kieu,Ma, H te,2015). In.Sin) in P. fluorescens SBW25 (De Bruijn Raaijmakers, 2009a), MassAandMassBC(massetolide Sin) in P. fluorescens SBW25 (De Bruijn Raaijmakers, 2009a), MassAandMassBC(massetolide)inP. fluorescens SS101 (De Bruijn Raaijmakers,2009b),ArfF(arthrofactin)inP. fluorescensMIS38(Washio etal.,2010),EtlR(entolysin)inP. entomophiliaL48T(Vallet- elyetal., G 2010),WlpR (WLIP) in P. putida RW10S2 (Rokni- adeh etal., 2012), Z XtlR(xantholysin)inP. putidaBW11M1(Lietal.,2013),andPcoRand RfiA(corpeptin)inP. corrugataCFBP5454(Stranoetal.,2015). In several Pseudomonasstrains,theprincipalregulatorofCLPbiosynthesisistheGacA/GacStwo- omponentsystemsinceamutation c inoneofbothencodinggenesleadstoalossinCLPproduction(De Bruijn Raaijmakers, 2009a). The GacA/GacS method is recognized to activate compact RNAs that bind to and sequester translational repressorproteins,whichblocktheribosomalbindingsitesinthemRNAof Gac- egulatedgenes.TwosmallRNAs(sRNAs)andtworepressorpror teins,RsmAandRsmE,havebeenlinkedtotheregulationofentolysin (Vallet- elyetal.,2010)andmassetolideAbiosynthesis(Song,Voort, G etal.,2015).InthemassetolideproducerP. fluorescensSS101,these repressorproteinsmostlikelyblocktranslationoftheLuxR- ypetrant scriptionalregulator,MassAR(Song,Voort,etal.,2015),bybindingto aspecificsitecalledtheGacAbox.Thissitecomprisesanontranslated leadersequenceupstreamoftheAUGcodononthemessengerRNA. In a number of CLP- roducing Pseudomonas strains, a GacA box is presp entupstreamtheLuxRregulatorsflankingtheCLPbiosynthesisgene clustersuggestingthatotherCLP- roducingPseudomonas strains may perhaps p show a equivalent regulation of lipopeptide biosynthesis (Song, Voort, etal.,2015).Besides the GacA/GacS regulatory program, N- cylhomoserine a lactone (N- HL)- ediated quorum sensing was shown to become reA m quiredforviscosinandputisolvin biosynthesis(Cui, Harling,Mutch, Darling,2005;Dubern,Lugtenberg, Bloemberg,2006)inP. fluorescensstrain5064andP. putidastrainPCL1445,althoughthisisnot the case in certain other Pseudomonasstrains(DeBruijnetal.,2008; Dumenyo,Mukherjee,Chun, Chatterjee,1998;Kinscherf Willis, 1999).InP. putidastrainPCL1445,twoheatshockproteinsDnaKand DnaJlocateddownstreamoftheGacsystemwereshowntoregulate putisolvinbiosynthesis(Dubern,Lagendijk,Lugtenberg, Bloemberg, 2005).RecentstudiesonthegeneticregulationofmassetolideAbiosynthesis in P. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/24876777 fluorescens SS101 revealed that the serine protease ClpP collectively with the chaperone ClpA regulates the biosynthesis of massetolidesvia a precise pathway involving the LuxR regulator (MassABC),theheatshockproteinsDnaKandDnaJ,andproteinsof the tricarboxylic acid (TCA) cycle (De Bruijn Raaijmakers, 2009b; Song,Aundy,vandeMortel, Raaijmakers,2014;Song,Sundqvist, etal.,2015).